![]() ![]() Detection and quantification of substances such as peptides, antibodies, hormones, proteins etc.For rapid presumptive screen of certain classes of drugs.Detection of potential allergens in the food samples.The concentration of serum antibodies can be determined in a virus test, e.g.Detection and measurement of antigen or antibody in the given sample.In this technique, however, the higher the concentration of antigen in the original sample, the lower is the absorbance.Enzyme-conjugated secondary antibody (Ab2) specific for the isotype of the primary antibody is added which is used to determine the amount of primary antibody bound to the well, as in indirect ELISA.The more the antigen present in the sample, the less free antibody will be available to bind to the antigen-coated well.In this technique, antibody is first incubated with the antigen (in the sample) in solution form and this mixture is then added to an antigen coated microtiter well.This method is used to detect the presence of serum antibodies against HIV, the causative agent of AIDS, which can be done within 6 weeks of infection.The amount of colored reaction product that forms is measured by specialized spectrophotometric plate readers, that measures the absorbance of all the wells used. ![]()
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